Cathelicidin Antimicrobial Peptide (cAMP) antibody

Details for Product No. ABIN350184
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Antigen
Synonyms CATH-2, CAP-18, CAP18, CRAMP, FALL-39, FALL39, LL37, Cnlp, Cramp, MCLP, CATHL7, CATH-B1, CATHB1, CATHL2
Reactivity
Human
(64), (26), (18), (6), (1), (1)
Host
Rabbit
(75), (10), (7), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(63), (34), (29), (19), (18), (11), (6), (6), (3), (2)
Pubmed 6 references available
Quantity 500 µg
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Catalog No. ABIN350184
454.67 $
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Immunogen A synthetic peptide from human Cathelicidin antimicrobial peptide conjugated to an immunogenic carrier protein has been used as the antigen.
Isotype IgG
Specificity Specific for Cathelicidin antimicrobial peptide.
Alternative Name LL-37
Background Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
Function: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
Subcellular location: Secreted.
Tissue specificity: Expressed in bone marrow and testis and neutrophils. Also known as: CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26.
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Cathelicidin Antimicrobial Peptide (cAMP) antibody Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18): IgG (ABIN350184), diluted 50 µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI.  The antibody selectively recognizes polymorphonuclar cells.
anti-Cathelicidin Antimicrobial Peptide (cAMP) antibody (2) WB on human neutrophil lysate using Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG (ABIN350184) at 50 µg/ml concentration. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
General Agerberth, Gunne, Odeberg et al.: "FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 1, pp. 195-9, 1995 (PubMed).

Cowland, Johnsen, Borregaard: "hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules." in: FEBS letters, Vol. 368, Issue 1, pp. 173-6, 1995 (PubMed).

Larrick, Hirata, Balint et al.: "Human CAP18: a novel antimicrobial lipopolysaccharide-binding protein." in: Infection and immunity, Vol. 63, Issue 4, pp. 1291-7, 1995 (PubMed).

Gudmundsson, Agerberth, Odeberg et al.: "The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes." in: European journal of biochemistry / FEBS, Vol. 238, Issue 2, pp. 325-32, 1996 (PubMed).

Larrick, Lee, Ma et al.: "Structural, functional analysis and localization of the human CAP18 gene." in: FEBS letters, Vol. 398, Issue 1, pp. 74-80, 1997 (PubMed).

Bals, Lang, Weiner et al.: "Rhesus monkey (Macaca mulatta) mucosal antimicrobial peptides are close homologues of human molecules." in: Clinical and diagnostic laboratory immunology, Vol. 8, Issue 2, pp. 370-5, 2001 (PubMed).

Validation Images
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