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Myeloperoxidase antibody (MPO)

Details for Product anti-MPO Antibody No. ABIN350505, Supplier: Log in to see
Antigen
  • fj80f04
  • LOC100335032
  • mKIAA4033
  • mpo
  • MPO
  • wu:fj80f04
Reactivity
Human
548
76
66
1
Host
Rabbit
313
262
11
2
1
1
Clonality
Polyclonal
Conjugate
This Myeloperoxidase antibody is un-conjugated
61
45
24
21
17
17
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
333
325
107
97
93
57
50
35
26
20
16
11
10
4
4
4
3
3
2
2
2
2
2
1
1
1
1
Supplier
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Immunogen Native myeloperoxidase isolated from human leucocytes
Isotype IgG
Specificity Specific for MPO.
Alternative Name MPO (MPO Antibody Abstract)
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
Subcellular location: Lysosome Also known as: MPO.
Application Notes A working concentration of 10-50 µg/ml is recommended.
The optimal dilution should be determined by the end user. This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Myeloperoxidase antibody (MPO) (ABIN350505) Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit a...
Immunohistochemistry (IHC) image for anti-Myeloperoxidase antibody (MPO) (ABIN350505) Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit a...
Western Blotting (WB) image for anti-Myeloperoxidase antibody (MPO) (ABIN350505) 10 µg of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred o...
Background publications Ramachandran, Boontheung, Xie, Sondej, Wong, Loo: "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." in: Journal of proteome research, Vol. 5, Issue 6, pp. 1493-503, 2006 (PubMed).

Sjöblom, Jones, Wood, Parsons, Lin, Barber, Mandelker, Leary, Ptak, Silliman, Szabo, Buckhaults, Farrell, Meeh, Markowitz, Willis, Dawson, Willson, Gazdar, Hartigan, Wu, Liu, Parmigiani, Park et al.: "The consensus coding sequences of human breast and colorectal cancers. ..." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Liu, Qian, Gritsenko, Camp, Monroe, Moore, Smith: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Fiedler, Davey, Fenna: "X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8 A resolution." in: The Journal of biological chemistry, Vol. 275, Issue 16, pp. 11964-71, 2000 (PubMed).

Hosokawa, Kawaguchi, Hikiji, Yamada, Suzuki, Nakagawa, Yoshihara, Yamaguchi: "Cloning and characterization of four types of cDNA encoding myeloperoxidase from human monocytic leukemia cell line, SKM-1." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 7, Issue 3, pp. 441-5, 1993 (PubMed).

Yamada, Yoshida, Hashinaka: "Identification of transcriptional cis-elements in introns 7 and 9 of the myeloperoxidase gene." in: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13479-85, 1993 (PubMed).

Hashinaka, Nishio, Hur, Sakiyama, Tsunasawa, Yamada: "Multiple species of myeloperoxidase messenger RNAs produced by alternative splicing and differential polyadenylation." in: Biochemistry, Vol. 27, Issue 16, pp. 5906-14, 1989 (PubMed).

Johnson, Nauseef, Care, Wheelock, Shane, Hudson, Koeffler, Selsted, Miller, Rovera: "Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species." in: Nucleic acids research, Vol. 15, Issue 5, pp. 2013-28, 1987 (PubMed).

Morishita, Kubota, Asano, Kaziro, Nagata: "Molecular cloning and characterization of cDNA for human myeloperoxidase." in: The Journal of biological chemistry, Vol. 262, Issue 8, pp. 3844-51, 1987 (PubMed).

Seto, Hirayu, Magnusson, Gestautas, Portmann, DeGroot, Rapoport: "Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase." in: The Journal of clinical investigation, Vol. 80, Issue 4, pp. 1205-8, 1987 (PubMed).