ERK1/2 antibody (pThr202, pTyr204)
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- Target See all ERK1/2 (MAPK1/3) Antibodies
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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Binding Specificity
- pThr202, pTyr204
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This ERK1/2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Specific for Erk1 and Erk2.
- Cross-Reactivity
- Human, Mouse, Rat
- Cross-Reactivity (Details)
- Other species not yet tested.
- Purification
- IgG
- Immunogen
- A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in many species including rat, mouse, zebra fish, xenopus and chicken.
- Isotype
- IgG
- Top Product
- Discover our top product MAPK1/3 Primary Antibody
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- Application Notes
- IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
- Handling Advice
- Avoid freeze and thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Expiry Date
- 12 months
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- Target
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- Alternative Name
- Erk1, Erk2 (MAPK1/3 Products)
- Synonyms
- erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
- Background
- FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF. SUBUNIT: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.,Protein Kinases,Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, MAPK1, PRKM1, PRKM2, MAPK3, PRKM3
- UniProt
- P27361
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