ERK1 / ERK2 (pThr202), (pTyr204) antibody

Details for Product No. ABIN350737
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, 9030612K14Rik, AA407128, AU018647, C78273, Erk2, Prkm1, p42mapk, erk, erk2, ert1, mapk, mapk1, mapk1a, mapk2, mpk1, prkm1, prkm2, ... show more
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, 9030612K14Rik, AA407128, AU018647, C78273, Erk2, Prkm1, p42mapk, erk, erk2, ert1, mapk, mapk1, mapk1a, mapk2, mpk1, prkm1, prkm2, xp42 show less
Epitope
pThr202, pTyr204
(66), (61), (18), (17), (12), (12), (8), (8), (8), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(215), (137), (131), (72), (66), (60), (36), (24), (9), (8), (8), (3), (2), (1), (1), (1), (1), (1)
Host
Sheep
(183), (36), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(169), (50), (48), (41), (40), (38), (21), (12), (11), (11), (3), (2), (2)
Pubmed 5 references available
Quantity 500 μg
Options
Shipping to United States (Change)
Availability Will be delivered in 7 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN350737
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in many species including rat, mouse, zebra fish, xenopus and chicken.
Isotype IgG
Specificity Specific for Erk1 and Erk2.
Alternative Name Erk1, Erk2
Background Function: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4).
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Activated by tyrosine phosphorylation in response to insulin and NGF.
Subunit: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH. Also known as: Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, MAPK1, PRKM1, PRKM2, MAPK3, PRKM3.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. This antibody may also recognise the unphosphorylated form.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Wissing, Jänsch, Nimtz et al.: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Validation Images
Did you look for something else?
back to top