Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Specificity
Specific for Pr3.
Cross-Reactivity
Human
Cross-Reactivity (Details)
Other species not yet tested.
Purification
IgG
Immunogen
A synthetic peptide from the c-terminal region of human Pr3 (Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen) conjugated to an immunogenic carrier protein was used as the immunogen.
PRTN3
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB, IHC, flow cytometry. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.,Signal Transduction,Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen, PR-3, P29, C-ANCA antigen, Neutrophil proteinase 4, MBN, PRTN3, NP-4