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Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody

Details for Product No. ABIN350906, Supplier: Login to see
Antigen
  • ric-4
  • ric4
  • sec9
  • snap-25
  • SNAP-25B
  • SNAP-25a
  • Bdr
  • SNAP-25
  • sp
  • RIC-4
  • RIC4
  • SEC9
  • SNAP
  • bA416N4.2
  • dJ1068F16.2
  • fj55h11
  • snap25
  • wu:fj55h11
Reactivity
Human
270
107
99
30
20
15
15
14
11
8
5
4
3
3
3
2
2
1
1
1
1
Host
Rabbit
145
127
24
7
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
7
7
7
7
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
232
150
95
65
51
45
27
17
16
11
11
8
2
2
2
1
Supplier
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Immunogen A synthetic peptide as a part of human Synaptosomal-associated protein 25 (SNAP-25, SNAP25, SEC9, RIC-4) conjugated to an immunogenic carrier protein was used as the immunogen.
Isotype IgG
Specificity Specific for SNAP-25.
Alternative Name SNAP 25 (SNAP25 Antibody Abstract)
Background Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
Subunit: Part of the SNARE core complex containingSNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1,SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.
Subcellular location: Membrane, Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation.
Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Also known as:SNAP-25, Synaptosomal-associated 25 kDa protein,SNAP25, RIC4, SEC9,SNAP, RIC-4, Super protein, SUP, FLJ23079, bA416N4.2, dJ1068F16.2.
Pathways
Application Notes A working concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Chen, Tomchick, Kovrigin et al.: "Three-dimensional structure of the complexin/SNARE complex." in: Neuron, Vol. 33, Issue 3, pp. 397-409, 2002 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Jagadish, Fernandez, Hewish et al.: "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." in: The Biochemical journal, Vol. 317 ( Pt 3), pp. 945-54, 1996 (PubMed).

Bark, Wilson: "Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25." in: Gene, Vol. 139, Issue 2, pp. 291-2, 1994 (PubMed).

Zhao, Hashida, Takahashi et al.: "Cloning and sequence analysis of the human SNAP25 cDNA." in: Gene, Vol. 145, Issue 2, pp. 313-4, 1994 (PubMed).