Synaptotagmin I (SYT1) antibody

Antigen: Synaptotagmin I (SYT1)

Synonyms: P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, D. Syt I, DSYT, DSYT2, SYT I, SYT1, SytI, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, l(2)k05909, syt-1, DmelCG3139, CG3139, Syti, GB20036, syt, svp65, DKFZp459D121, DKFZP459P193, p65

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus), Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350923

Additional Information

Alternative name: Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65)

UniProt: P21707

Immunogen: A synthetic peptide as a part of rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues. Also known as: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65.

Specificity: Appears to be specific for SYTI.

Synonyms: P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, D. Syt I, DSYT, DSYT2, SYT I, SYT1, SytI, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, l(2)k05909, syt-1, DmelCG3139, CG3139, Syti, GB20036, syt, svp65, DKFZp459D121, DKFZP459P193, p65

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Sutton, Davletov, Berghuis et al.: "Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold." in: Cell, Vol. 80, Issue 6, pp. 929-38, 1995 (PubMed).

Schivell, Batchelor, Bajjalieh: "Isoform-specific, calcium-regulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27770-5, 1996 (PubMed).

Shao, Fernandez, Suedhof et al.: "Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?" in: Biochemistry, Vol. 37, Issue 46, pp. 16106-15, 1998 (PubMed).

Coppola, Magnin-Luthi, Perret-Menoud et al.: "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32756-62, 2001 (PubMed).

Fernandez, Arauc, Ubach et al.: "Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine." in: Neuron, Vol. 32, Issue 6, pp. 1057-69, 2001 (PubMed).

Heindel, Schmidt, Veit: "Palmitoylation sites and processing of synaptotagmin I, the putative calcium sensor for neurosecretion." in: FEBS letters, Vol. 544, Issue 1-3, pp. 57-62, 2003 (PubMed).

Schivell, Mochida, Kensel-Hammes et al.: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, 2005 (PubMed).