IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. Acts as a negative growth regulator. SUBCELLULAR LOCATION: Cytoplasm. Membrane, Peripheral membrane protein. Nucleus. Note: Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus.,Vesicle Transport,Tumor susceptibility gene 101 protein