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VAMP3 antibody (Vesicle-Associated Membrane Protein 3)

Details for Product anti-VAMP3 Antibody No. ABIN351362, Supplier: Log in to see
Antigen
  • ceb
  • CEB
  • D130027G05Rik
  • VAMP-3
  • vamp-3
  • wu:fj59a03
  • zgc:73378
  • zgc:86726
Reactivity
Human, Rat (Rattus), Mouse (Murine)
74
37
15
2
Host
Rabbit
44
30
3
Clonality
Polyclonal
Conjugate
This VAMP3 antibody is un-conjugated
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
71
46
20
7
6
4
2
1
1
Supplier
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Immunogen A synthetic peptide from human VAMP3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Isotype IgG
Specificity Specific for VAMP3.
Alternative Name VAMP3 (VAMP3 Antibody Abstract)
Background Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.
Function: Trafficking protein from a constitutively recycling pathway.
Subcellular location: Membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Also known as: VAMP-3, VAMP3, Synaptobrevin-3, Cellubrevin, SYB3, CEB.
Application Notes A working concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Carninci, Kasukawa, Katayama, Gough, Frith, Maeda, Oyama, Ravasi, Lenhard, Wells, Kodzius, Shimokawa, Bajic, Brenner, Batalov, Forrest, Zavolan, Davis, Wilming, Aidinis, Allen, Ambesi-Impiombato et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Gevaert, Goethals, Martens, Van Damme, Staes, Thomas, Vandekerckhove: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Yang, Mora, Ryder, Coker, Hansen, Allen, Pessin: "VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking." in: Molecular and cellular biology, Vol. 21, Issue 5, pp. 1573-80, 2001 (PubMed).

Bernstein, Whiteheart: "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." in: Blood, Vol. 93, Issue 2, pp. 571-9, 1999 (PubMed).

McMahon, Ushkaryov, Edelmann, Link, Binz, Niemann, Jahn, Suedhof: "Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein." in: Nature, Vol. 364, Issue 6435, pp. 346-9, 1993 (PubMed).