ENPEP antibody (AA 805-818)

Catalog No. ABIN351480

The Goat Polyclonal anti-ENPEP antibody (ABIN351480) specifically detects ENPEP in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow and Monkey samples.

Quick Overview for ENPEP antibody (AA 805-818) (ABIN351480)

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPEP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENPEP Antibody

Binding Specificity: AA 805-818

Specificity: Human ENPEP.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine (100%) Dog (93%) Panda, Bat, Rabbit, Horse, Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EQYQKTSLAQEKEK from an internal region of human ENPEP (NP_001968.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine (100%), Dog (93%), Panda, Bat, Rabbit, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: ENPEP / Aminopeptidase A

Background: Name/Gene ID: ENPEP
Subfamily: Metallopeptidase M1
Family: Exopeptidase

Synonyms: ENPEP, APA, Aminopeptidase A, AP-A, CD249 antigen, Differentiation antigen gp160, EAP, Glutamyl aminopeptidase, gp160, CD249

Gene ID: 2028

NCBI Accession: NP_001968

UniProt: Q07075

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones