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ERK1/2 antibody

MAPK1/3 Reactivity: Human, Mouse, Rat, Hamster WB, ELISA, IHC, IHC (p), IF, FACS, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN351562
  • Target See all ERK1/2 (MAPK1/3) Antibodies
    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
    Reactivity
    • 192
    • 162
    • 159
    • 23
    • 23
    • 18
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Hamster
    Host
    • 143
    • 51
    • 4
    Rabbit
    Clonality
    • 139
    • 59
    Polyclonal
    Conjugate
    • 97
    • 12
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    This ERK1/2 antibody is un-conjugated
    Application
    • 155
    • 65
    • 51
    • 50
    • 49
    • 37
    • 24
    • 22
    • 21
    • 13
    • 4
    • 3
    • 3
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product MAPK1/3 Primary Antibody
  • Application Notes
    Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P, IP (1:50), WB (1:1000)

    Usage: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25.
    Comment

    Target Species of Antibody: Rat

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
    Preservative
    Without preservative
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
    Alternative Name
    ERK1 + ERK2 (MAPK1/3 Products)
    Synonyms
    erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
    Background
    Name/Gene ID: ERK1/ERK2

    Synonyms: ERK1/ERK2

    This antibody detects: ERK1 + ERK2
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