CD3 antibody (Biotin)

Catalog No. ABIN356215

Product Details anti-CD3 Antibody (Biotin)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody is specific to CD3e (Mr. 25 kDa). The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Clone: C363-29B (YCD3-1)

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Identification and enumeration of CD3+ cells (< / = 3 μg/10^6 cells). (Ref.1-2)Immunoprecipitation. Functional Assay: In vitro Depletion of CD3+ cells. In vitro Activation of T cells (accessory cells must be provided). (Ref.1,3,4)Immunohistochemistry. (Ref.5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Synonyms: CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway