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Protein Arginine Methyltransferase 5 (PRMT5) (C-Term) antibody

Details for Product No. ABIN356529, Supplier: Log in to see
Antigen
  • SKB1
  • DDBDRAFT_0187422
  • DDBDRAFT_0235403
  • DDB_0187422
  • DDB_0235403
  • NV18830
  • hsl7
  • jbp1
  • skb1
  • ibp72
  • skb1hs
  • hrmt1l5
  • Prmt5
  • HRMT1L5
  • IBP72
  • JBP1
  • SKB1Hs
  • si:zc14a17.2
  • wu:fb95f10
  • zgc:110669
  • Skb1
  • Jbp1
Epitope
C-Term
31
22
15
6
5
4
3
2
2
2
2
2
2
1
Reactivity
Human
134
38
37
8
4
4
3
1
1
1
1
1
Host
Rabbit
112
14
12
Clonality
Polyclonal
Conjugate
Un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
57
52
17
15
11
10
4
3
2
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRMT5.
Isotype Ig
Specificity This antibody is specific to PRMT5 (C-term).
Purification Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PRMT5 (PRMT5 Antibody Abstract)
Background Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.Synonyms: 72 kDa ICln-binding protein, HRMT1L5, Histone-arginine N-methyltransferase PRMT5, IBP72, JBP1, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, SKB1, SKB1Hs
Gene ID 10419, 5874
UniProt O14744
Research Area Chromatin and Nuclear Signaling
Application Notes ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Protein Arginine Methyltransferase 5 (PRMT5) (C-Term) antibody (ABIN356529) anti-Protein Arginine Methyltransferase 5 (PRMT5) (C-Term) antibody
 image for anti-Protein Arginine Methyltransferase 5 (PRMT5) (C-Term) antibody (ABIN356529) anti-Protein Arginine Methyltransferase 5 (PRMT5) (C-Term) antibody (Image 2)
Background publications Pal, Yun, Datta et al.: "mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7475-87, 2003 (PubMed).

Rho, Choi, Seong et al.: "Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 11393-401, 2001 (PubMed).

Pollack, Kotenko, He et al.: "The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31531-42, 1999 (PubMed).

Gilbreth, Yang, Bartholomeusz et al.: "Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14781-6, 1999 (PubMed).