SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (Middle Region) antibody

Details for Product No. ABIN356694
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms KMT7, SET7, SET7/9, SET9, set7, zgc:101860, zgc:92330, 1600028F23Rik, H3K4MT, Set7, Set7/9, mKIAA1717
Epitope
Middle Region
(10), (7), (4), (4), (3), (3), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(64), (18), (16), (13), (12), (12), (1), (1)
Host
Rabbit
(43), (23)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (31), (16), (10), (8), (6), (5), (2), (2)
Pubmed 5 references available
Quantity 0.4 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN356694
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This SET7/9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human SET9.
Specificity This antibody is specific to SET7 (SET9) (Center).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETD7 / SET9
Background Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
Alternate names: H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N- methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain- containing protein 7, SET7, SET7/9
Gene ID 80854, 9606
UniProt Q8WTS6
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (Middle Region) antibody anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (Middle Region) antibody
anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (Middle Region) antibody (2) anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (Middle Region) antibody (Image 2)
Background publications Nishioka, Chuikov, Sarma et al.: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, 2002 (PubMed).

Wilson, Jing, Walker et al.: "Crystal structure and functional analysis of the histone methyltransferase SET7/9." in: Cell, Vol. 111, Issue 1, pp. 105-15, 2002 (PubMed).

Kwon, Chang, Kwak et al.: "Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet." in: The EMBO journal, Vol. 22, Issue 2, pp. 292-303, 2003 (PubMed).

Xiao, Jing, Wilson et al.: "Structure and catalytic mechanism of the human histone methyltransferase SET7/9." in: Nature, Vol. 421, Issue 6923, pp. 652-6, 2003 (PubMed).

Wysocka, Myers, Laherty et al.: "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." in: Genes & development, Vol. 17, Issue 7, pp. 896-911, 2003 (PubMed).

Validation Images
Did you look for something else?
back to top