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SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody

Details for Product No. ABIN356695, Supplier: Login to see
Antigen
  • KMT7
  • SET7
  • SET7/9
  • SET9
  • set7
  • zgc:101860
  • zgc:92330
  • 1600028F23Rik
  • H3K4MT
  • Set7
  • Set7/9
  • mKIAA1717
Epitope
C-Term
11
8
5
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
89
25
21
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
60
29
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
66
35
20
14
11
8
7
4
3
3
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SET7.
Isotype Ig
Specificity This antibody is specific to SET7 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETD7 / SET9 (SETD7 Antibody Abstract)
Background Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function, rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.Synonyms: H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7, SET7/9
Gene ID 80854, 9606
UniProt Q8WTS6
Pathways
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody (ABIN356695) anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody
Background publications Wysocka, Myers, Laherty et al.: "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." in: Genes & development, Vol. 17, Issue 7, pp. 896-911, 2003 (PubMed).

Xiao, Jing, Wilson et al.: "Structure and catalytic mechanism of the human histone methyltransferase SET7/9." in: Nature, Vol. 421, Issue 6923, pp. 652-6, 2003 (PubMed).

Kwon, Chang, Kwak et al.: "Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet." in: The EMBO journal, Vol. 22, Issue 2, pp. 292-303, 2003 (PubMed).

Wilson, Jing, Walker et al.: "Crystal structure and functional analysis of the histone methyltransferase SET7/9." in: Cell, Vol. 111, Issue 1, pp. 105-15, 2002 (PubMed).

Nishioka, Chuikov, Sarma et al.: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, 2002 (PubMed).