SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody

Details for Product No. ABIN356695
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Antigen
Synonyms KMT7, Set7, H3K4MT, Set7/9, mKIAA1717, 1600028F23Rik, SET7, SET7/9, SET9, set7, zgc:101860, zgc:92330
Epitope
C-Term
(3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(58), (18), (16), (13), (12), (12), (1), (1)
Host
Rabbit
(34), (25)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(43), (24), (18), (10), (5), (4), (2), (2), (2), (2), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN356695
Quantity 0.4 mL
Price
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SET7.
Specificity This antibody is specific to SET7 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETD7 / SET9
Background Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post- translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function, rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.
Alternate names: H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N- methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain- containing protein 7, SET7, SET7/9
Gene ID 80854
NCBI Accession 9606
UniProt Q8WTS6
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody anti-SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (C-Term) antibody
Background publications Nishioka, Chuikov, Sarma et al.: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, 2002 (PubMed).

Wilson, Jing, Walker et al.: "Crystal structure and functional analysis of the histone methyltransferase SET7/9." in: Cell, Vol. 111, Issue 1, pp. 105-15, 2002 (PubMed).

Kwon, Chang, Kwak et al.: "Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet." in: The EMBO journal, Vol. 22, Issue 2, pp. 292-303, 2003 (PubMed).

Xiao, Jing, Wilson et al.: "Structure and catalytic mechanism of the human histone methyltransferase SET7/9." in: Nature, Vol. 421, Issue 6923, pp. 652-6, 2003 (PubMed).

Wysocka, Myers, Laherty et al.: "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." in: Genes & development, Vol. 17, Issue 7, pp. 896-911, 2003 (PubMed).

Hosts (34), (25)
Reactivities (58), (18), (16), (13), (12), (12), (1), (1)
Applications (43), (24), (18), (10), (5), (4), (2), (2), (2), (2), (2), (2), (1)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1), (1), (1), (1), (1), (1)
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