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Antibodies
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anti-BCL2-Associated X Protein (BAX) (BH3 Domain) antibody
BCL2-Associated X Protein (BAX) (BH3 Domain) antibody
| Antigen |
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| Synonyms |
BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (90), Immunofluorescence (IF) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunoprecipitation (IP) (37), Flow Cytometry (FACS) (36), ELISA (18), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN356811 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein
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Alternative name
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Bax
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Gene ID
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581
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Swiss-Prot
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Q07812
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Immunogen
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This antibody was developed against a synthetic peptide selected from the N-terminalregion (BH3 domain) of human Bax.
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Cross-Reactivity
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Human, Mouse (Murine)
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Isotype
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Ig (Matching secondary antibodies)
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Description
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Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimersand act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellularactivities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. Thisprotein is reported to interact with, and increase the opening of, the mitochondrialvoltage-dependent anion channel (VDAC), which leads to the loss in membrane potentialand the release of cytochrome c. The expression of the Bax gene is regulated by the tumorsuppressor P53 and has been shown to be involved in P53-mediated apoptosis.
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Specificity
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This antibody is specific to Bax (BH3 Domain). Species: Human. Others not tested. Add. Information: Calculated MW: 24220 Da
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Application Notes
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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PBS containing 0.09% (W/V) Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
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Restrictions
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For Research Use only
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Alternatives for antigen "BCL2-Associated X Protein (BAX)", type "Antibodies"
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Hosts
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Mouse (78), Rabbit (68), Sheep (3)
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Reactivities
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Human (131), Mouse (Murine) (69), Rat (Rattus) (65), Dog (Canine) (32), Cow (Bovine) (31), Pig (Porcine) (31), Sheep (Ovine) (30), Chicken (1), Horse (Equine) (1), Rabbit (1)
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Applications
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Western Blotting (WB) (90), Immunofluorescence (IF) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunoprecipitation (IP) (37), Flow Cytometry (FACS) (36), ELISA (18), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Biotin (8), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (2), universal (1)
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