Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody

Details for Product No. ABIN357069
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Synonyms DKK-2, Dkk4
(23), (8), (5), (4), (1), (1), (1), (1), (1), (1), (1)
(55), (17), (8), (3), (3), (1)
(46), (16)
(6), (5), (4), (3), (3), (3)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (40), (15), (6), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DKK2.
Isotype Ig
Specificity This antibody detects DKK2 at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name Dickkopf-2 (DKK2) (DKK2 Antibody Abstract)
Background The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.Synonyms: Dickkopf-related protein 2, Dkk-2
Molecular Weight 28447 Da
Gene ID 27123, 9606
UniProt Q9UBU2
Pathways WNT Signaling
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Image no. 1 for anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody (ABIN357069) anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody
Image no. 2 for anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody (ABIN357069) anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody (Image 2)
Image no. 3 for anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody (ABIN357069) anti-Dickkopf 2 Homolog (Xenopus Laevis) (DKK2) (N-Term) antibody (Image 3)
Background publications Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Brott, Sokol: "Regulation of Wnt/LRP signaling by distinct domains of Dickkopf proteins." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6100-10, 2002 (PubMed).

Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).

Catalog No. ABIN357069
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