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DKK3 (N-Term) antibody

Details for Product No. ABIN357071, Supplier: Log in to see
Antigen
  • AW061014
  • C87148
  • REIC
  • RIG
  • Reic
  • p29
  • dickkopf 3 homolog (Xenopus laevis)
  • dickkopf homolog 3 (Xenopus laevis)
  • dickkopf WNT signaling pathway inhibitor 3
  • DKK3
  • Dkk3
Epitope
N-Term
37
20
16
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
137
51
35
9
1
1
1
1
1
1
1
1
1
Host
Rabbit
100
30
22
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
105
92
75
30
15
13
5
4
3
3
1
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DKK3.
Isotype Ig
Specificity This antibody detects DKK3 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name Dickkopf-3 (DKK3)
Background DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
Molecular Weight 38291 Da
Gene ID 27122
NCBI Accession NP_001018067
UniProt Q9UBP4
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-DKK3 (N-Term) antibody (ABIN357071) anti-DKK3 (N-Term) antibody
 image for anti-DKK3 (N-Term) antibody (ABIN357071) anti-DKK3 (N-Term) antibody (Image 2)
 image for anti-DKK3 (N-Term) antibody (ABIN357071) anti-DKK3 (N-Term) antibody (Image 3)
Background publications Clark, Gurney, Abaya, Baker, Baldwin, Brush, Chen, Chow, Chui, Crowley, Currell, Deuel, Dowd, Eaton, Foster, Grimaldi, Gu, Hass, Heldens, Huang, Kim, Klimowski, Jin, Johnson, Lee, Lewis, Liao, Mark et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Krupnik, Sharp, Jiang, Robison, Chickering, Amaravadi, Brown, Guyot, Mays, Leiby, Chang, Duong, Goodearl, Gearing, Sokol, McCarthy: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).