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Details for Product No. ABIN358462

DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (pSer154) antibody

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Antigen
Synonyms
ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSF ... show more
Epitope
»Alternatives pSer154
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA
Pubmed 2 references available
Catalog no. ABIN358462
Quantity 0.4 mL
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S154 of human Dnmt1.
Specificity This antibody detects Dnmt1 pSer154.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Alternative Name DNMT1
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.
Alternate names: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT
Molecular Weight 183165 Da
Gene ID 1786
NCBI Accession 9606
UniProt P26358
Research Area DNA/RNA
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Dion, Lin, Hubert et al.: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." in: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, 2008 (PubMed).

Liao, Siu, Chan et al.: "Hypermethylation of RAS effector related genes and DNA methyltransferase 1 expression in endometrial carcinogenesis." in: International journal of cancer. Journal international du cancer, Vol. 123, Issue 2, pp. 296-302, 2008 (PubMed).

Alternatives for antigen "DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)", type "Antibodies"
Hosts (132), (18), (10), (9), (2)
Reactivities (161), (64), (58), (52), (43), (32), (24), (8), (2), (1), (1), (1), (1), (1), (1), (1)
Applications (103), (50), (50), (40), (30), (14), (12), (9), (8), (7), (5), (1), (1)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (15), (13), (12), (12), (8), (7), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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