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DOK2 (C-Term) antibody

Details for Product No. ABIN359960
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Antigen
Synonyms DokR, Frip, Dok-2, p56DOK, p56dok-2
Epitope
C-Term
(35), (24), (13), (12), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(134), (66), (49), (48), (40), (37), (24), (2)
Host
Rabbit
(127), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(87), (43), (40), (32), (31), (25), (7), (5)
Pubmed 2 references available
Quantity 0.4 mL
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Catalog No. ABIN359960
357.50 $
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DOK2.
Specificity This antibody reacts to DOK2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name DOK2 / p56 Dok2
Background Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. Dok-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. Dok-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the Dok1 and Dok2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the Dok proteins serve as mediators of bcr-abl signaling. The role of Dok proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9,22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.
Alternate names: Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), p56Dok-2
Gene ID 9046
NCBI Accession 9606
UniProt O60496
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-DOK2 (C-Term) antibody anti-DOK2 (C-Term) antibody
anti-DOK2 (C-Term) antibody (2) anti-DOK2 (C-Term) antibody (Image 2)
Background publications Di Cristofano, Carpino, Dunant et al.: "Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4827-30, 1998 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Validation Images
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