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NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1173-1202) antibody

Details for Product No. ABIN360536
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Antigen
Synonyms D8Ertd790e, kat, NY-REN-55, SRPS2, SRPS2A, ATNEK1, F20D21.32, F20D21_32, NIMA-RELATED KINASE1, NIMA-related serine/threonine kinase 1
Epitope
C-Term, AA 1173-1202
(1), (1)
Reactivity
Hamster, Human
(13), (11), (11), (11), (11), (1)
Host
Rabbit
(14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(9), (5), (4), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN360536
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Immunogen KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.
Specificity This antibody is specific to NEK1.
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name NEK1
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Alternate names: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY- REN-55, Serine/threonine-protein kinase Nek1
Gene ID 4750
NCBI Accession NP_001186326.1
UniProt Q96PY6
Application Notes ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1173-1202) antibody anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1173-1202) antibody
anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1173-1202) antibody (2) anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1173-1202) antibody (Image 2)
Background publications Letwin, Mizzen, Motro et al.: "A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells." in: The EMBO journal, Vol. 11, Issue 10, pp. 3521-31, 1992 (PubMed).

Scanlan, Gordan, Williamson et al.: "Antigens recognized by autologous antibody in patients with renal-cell carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 83, Issue 4, pp. 456-64, 1999 (PubMed).

Surpili, Delben, Kobarg: "Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1." in: Biochemistry, Vol. 42, Issue 51, pp. 15369-76, 2003 (PubMed).

Validation Images
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