PTPN5 antibody (N-Term)
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- Target See all PTPN5 Antibodies
- PTPN5 (Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PTPN5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody reacts to STEP.
- Purification
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human STEP.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product PTPN5 Primary Antibody
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- Application Notes
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- PTPN5 (Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5))
- Alternative Name
- PTPN5 / STEP (PTPN5 Products)
- Synonyms
- STEP antibody, Step antibody, PTPSTEP antibody, protein tyrosine phosphatase, non-receptor type 5 antibody, ptpn5 antibody, PTPN5 antibody, Ptpn5 antibody
- Background
- STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90 % identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.Synonyms: FLJ14427, Neural-specific protein-tyrosine phosphatase, STEP20, STEP38, STEP46, STEP61, Striatum-enriched protein-tyrosine phosphatase, Tyrosine-protein phosphatase non-receptor type 5
- Gene ID
- 84867, 9606
- UniProt
- P54829
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