SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) antibody

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1)

Synonyms: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: Western Blotting (WB)

Catalog no.: ABIN361272

Additional Information

Alternative name: Small ubiquitin-like modifier protein 1 (SUMO1)

Immunogen: recombinant proSUMO1 from Arabidopsis thaliana Q547B9

Format: Lyophilized

Description: SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversibleway to various protein targets and plays a role as a signaling regulator.

Specificity: Predicted reactivity: dicots including: Glycine max, Lycopersicum esculentum, Nicotiana tabacum,Pisum sativum, monocots including: Oryza sativa, Zea mays, trees: Piceasitchensis, Populus trichocarpa.

Synonyms: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Application Details

Application Notes: Recommended Dilution 1: 1000 - 1: 5000 for standard ECL (WB).

Buffer: Total, purified IgG

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

20 ug of Arabidopsis thaliana total cell extract HA-proSUMO1 (HA-epitope andpro-Small Ubiquitinlike MOdifierprotein1 (1), empty vector only (2), wereseparated on 15%gel, SDS -PAGE and blotted on PVDF membrane. Filters were blocked in 5%milk for 1h,incubated with 1: 1000 anti-AtSUMO1antibody (AS08 308)for 1 h, followed by incubation with 1: 15000 secondary anti-rabbit antibodies(1h) coupled withHRP andvisualization (10 seconds exposure)with standard ECL. Note: signal in the empty vector lane comes from endogenous SUMO1detected by the antibody.