Ubiquitin C Terminal Hydrolase 1 (UCHL1) antibody

Antigen: Ubiquitin C Terminal Hydrolase 1 (UCHL1)

Synonyms: PARK5, PGP95, PGP9.5, Uch-L1, cb358, wu:fc55h08, UCHL1, UCH-L1, park5, pgp9.5, uch-l1, MGC133697, MGC132191, uchl1

Clonality: Polyclonal

Host: Chicken

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361370

Additional Information

Format: Liquid

Description: Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Anti-UCHL1

Specificity: Specific for the ~24kDa UCHL1 protein. It is expected that it will work on other mammalian tissues.

Application Details

Application Notes: Recommended Dilution: WB: 1:2,500 IF: 1:500 Quality Control: Western blots performed on each lot.

Purification: Total IgY fraction

Buffer: contains 10mM sodium azide

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein.

Rat mixed neuron/glial cultures stained with anti-UCHL1 antibody (green) and rabbit anti-GFAP antibody (620-GFAP) (red). Blue is a DNA stain.