Tyrosine Hydroxylase (TH) antibody

Antigen: Tyrosine Hydroxylase (TH)

Synonyms: TH, TyHyd, GB15303, th, LOC100219119, LOC446095, 2nd copy, Th, TYH, DYT14, DYT5b, The, DH65B, DTH, Pale, VII, dTH65B, pale/ple, DmelCG10118, CG10118

Clonality: Polyclonal

Host: Sheep

Reactivity: Mammalian

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361414

Additional Information

Alternative name: Tyrosine Hydroxylase

Format: Affinity-purified

Description: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001). Anti-Tyrosine Hydroxylase Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.

Specificity: Specific for the ~60k tyrosine hydroxylase protein.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IF (frozen sections, unpublished observations): 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.

Storage: Store at -20 °C, stable for 1 year

Research Area: Cancer, Amino Acids, Enzymes

Restrictions: For Research Use only

Images

Western blots of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.