Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) (pSer880) antibody

Antigen: Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)

Synonyms: GLUR2, GLURB, HBGR2, GluR-K2, GRIA2, GluR-2, GluR-B, MGC139795, ATGLR2, ATGLR3.1, F5J6.2, F5J6_2, GLR2, GLR3.1, GLUTAMATE RECEPTOR 2, ATGLR3.2, ATGLUR2, F23E12.150, F23E12_150, GLR3.2, GLUTAMATE RECEPTOR 3.2, DKFZp459F083, GLUH1, GLUR1, GLURA, HBGR1, MGC133252, Glr1, Glr-1, GluA1, GluRA, Glur1, HIPA1, GluR-A, Glur-1, AI853806, 2900051M01Rik, gluR-A, GluA2, Glur2, Glur-2, GluR2, gluR-B

Epitope: pSer880

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361455

Additional Information

Alternative name: Phospho Ser880 GluR2

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser880 of rat GluR2.

Format: Affinity-purified

Description: Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by (-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Kein nen et al., 1990, Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999) Anti-Phospho-Ser880 GluR2 Western blot of rat brain homogenate showing specific immunolabeling of the ~100k GluR2 protein phosphorylated at Ser880 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Specificity: The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will react with chicken, human, mouse, non-human primate and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for ~100k phosphorylated at Ser880.

Synonyms: GLUR2, GLURB, HBGR2, GluR-K2, GRIA2, GluR-2, GluR-B, MGC139795, ATGLR2, ATGLR3.1, F5J6.2, F5J6_2, GLR2, GLR3.1, GLUTAMATE RECEPTOR 2, ATGLR3.2, ATGLUR2, F23E12.150, F23E12_150, GLR3.2, GLUTAMATE RECEPTOR 3.2, DKFZp459F083, GLUH1, GLUR1, GLURA, HBGR1, MGC133252, Glr1, Glr-1, GluA1, GluRA, Glur1, HIPA1, GluR-A, Glur-1, AI853806, 2900051M01Rik, gluR-A, GluA2, Glur2, Glur-2, GluR2, gluR-B

Application Details

Application Notes: WB: 1:1000.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Buffer: 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage: Store at –20°C; stable for at least one year.

Restrictions: For Research Use only

Images

Western blots of rat brain homogenate showing specific immunolabeling of the ~100k GluR2 protein phosphorylated at Ser880 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).