GRIA2 antibody (AA 25-360)

Catalog No. ABIN3043838

Product Details anti-GRIA2 Antibody

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Binding Specificity: AA 25-360

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Glutamate receptor 2(GRIA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Glutamate receptor 2(GRIA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutamate receptor, ionotropic, AMPA 2
Protein Name: Glutamate receptor 2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, The detection limit for GRIA2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GRIA2 antibody (ABIN3043838)

Liu, Mei, Li, Roboti, Pang, Ying, Gao, Lowe, Bao: "Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 2, pp. 346-351, (2018) (PubMed).

Dai, Li, Zhong, Chen, Cheng, Liu, Li: "The paracrine effect of cobalt chloride on BMSCs during cognitive function rescue in the HIBD rat." in: Behavioural brain research, Vol. 332, pp. 99-109, (2017) (PubMed).

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GRIA2 (GRIA2 Products)

Synonyms: GLUR2 antibody, GLURB antibody, GluA2 antibody, GluR-K2 antibody, HBGR2 antibody, GluR-2 antibody, GluR-B antibody, Glur-2 antibody, Glur2 antibody, GluR2 antibody, gluR-B antibody, glur2a antibody, gria2.1 antibody, zfGRIA2[a] antibody, glur2 antibody, glutamate ionotropic receptor AMPA type subunit 2 antibody, glutamate receptor, ionotropic, AMPA 2 antibody, glutamate receptor, ionotropic, AMPA2 (alpha 2) antibody, glutamate receptor, ionotropic, AMPA 2a antibody, glutamate receptor, ionotropic, AMPA 2 S homeolog antibody, GRIA2 antibody, Gria2 antibody, gria2a antibody, gria2.S antibody

Background: Glutamate receptor 2, also known as GLUR2, is a protein that in humans is encoded by the GRIA2 gene. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. GLUR2's cytogenetic location is 4q32.1. The crystal structures of the GLUR2 ligand-binding core in the apo state and in the presence of the antagonist DNQX, the partial agonist kainate, and the full agonists AMPA and glutamate.GLUR2 plays a major role in depression at synapses in which glutamate remains in the synaptic cleft for prolonged periods of time during normal operation of the synapse. The overexpression of GLUR2 increases dendritic spine size and density in hippocampal neurons, and more remarkably, induces spine formation in GABA-releasing interneurons that normally lack spines.

Synonyms: AMPA 2 antibody|AMPA selective glutamate receptor 2 antibody|AMPA-selective glutamate receptor 2 antibody|AMPA2 antibody|GluA2 antibody|GLUR 2 antibody|GLUR B antibody|GluR K2 antibody|GluR-2 antibody|GluR-B antibody|GluR-K2 antibody|GLUR2 antibody|GLURB antibody|Glutamate receptor 2 antibody|Glutamate receptor ionotropic AMPA 2 antibody|Glutamate receptor ionotropic antibody|Gria2 antibody|GRIA2_HUMAN antibody|HBGR2 antibody

Gene ID: 14800

UniProt: P42262

Pathways: PI3K-Akt Signaling