Polo-Like Kinase 1 (PLK1) (pThr210) antibody

Antigen: Polo-Like Kinase 1 (PLK1)

Synonyms: PLK, STPK13, Snk, plk, MGC52566, Plx1, PLK-1, stpk13, MGC69251, MGC133927

Epitope: pThr210

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Human

Application: Western Blotting (WB)

Catalog no.: ABIN361457

Additional Information

Alternative name: Phospho-Thr210 Polo-like Kinase 1

Format: Affinity-purified

Description: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK?s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005). Anti-Phospho-Thr210 PLK1 Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for ~66k PLK phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by (-phosphatase treatment.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.