Gap Junction Protein, alpha 1, 43kd (GJA1) (pSer368) antibody

Antigen: Gap Junction Protein, alpha 1, 43kd (GJA1)

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Epitope: pSer368

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361460

Additional Information

Alternative name: Phospho-Ser368 Connexin43

Format: Affinity-purified

Description: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b). Anti-Phospho-Ser368 Connexin43 Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment with (-Ptase.

Specificity: Specific for the ~43k connexin43 protein phosphorylated at Ser368. Immunolabeling is blocked by -phosphatase treatment.

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment with (-Ptase.