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CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133)

Details for Product anti-CREB1 Antibody No. ABIN361461, Supplier: Log in to see
Antigen
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Alternatives
anti-Rat (Rattus) CREB1 antibody for Enzyme Immunoassay
Epitope
pSer133
112
38
36
35
20
18
17
16
15
15
8
7
7
6
5
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
509
327
293
41
41
36
29
27
25
24
21
14
13
10
7
7
3
1
1
1
1
1
1
Host
Rabbit
424
84
11
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
17
14
14
7
6
6
6
5
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Western Blotting (WB)
405
212
181
83
83
44
42
30
29
11
11
10
4
3
3
2
1
1
1
1
Options
Supplier
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Available images

Specificity Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by (-phosphatase treatment.
Purification Affinity-purified
Alternative Name CREB (CREB1 Antibody Abstract)
Background It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002). Anti-Phospho-Ser133 CREB Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase ((-Ptase, 1200 units for 30 min).
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN361461) Western blots of a forskolin stimulated rat hippocampal lysate showing specific immun...
Product cited in: Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, 2011 (PubMed).