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GRIA1 antibody (Glutamate Receptor, Ionotropic, AMPA 1) (pSer845)

Details for Product anti-GRIA1 Antibody No. ABIN361477, Supplier: Log in to see
Antigen
  • 2900051M01Rik
  • Glr-1
  • Glr1
  • GluA1
  • GLUH1
  • Glur-1
  • gluR-A
  • GluR-A
  • gluR-K1
  • glur1
  • Glur1
  • GLUR1
  • GLURA
  • GluRA
  • GRIA1
  • HBGR1
  • HIPA1
Alternatives
anti-Rat (Rattus) GRIA1 antibody for Western Blotting
Epitope
pSer845
45
36
28
26
22
21
14
13
13
13
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
200
196
162
8
7
6
3
2
2
Host
Rabbit
212
50
5
Clonality
Polyclonal
Conjugate
This GRIA1 antibody is un-conjugated
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4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
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2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
199
81
52
45
41
40
26
8
5
3
3
1
1
1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.
Specificity The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with λ-Ptase.
Purification Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name GluR1 (GRIA1 Antibody Abstract)
Background Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as arning and memory (Lee at al., 2003; Esteban at al., 2003).
Pathways PI3K-Akt Signaling
Application Notes WB: 1:1000. IHC: 1:1000 (frozen sections).
Restrictions For Research Use only
Format Liquid
Buffer 100 myl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 myg per ml BSA and 50% glycerol.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-GRIA1 antibody (Glutamate Receptor, Ionotropic, AMPA 1) (pSer845) (ABIN361477) Western blots of rat hippocampal lysate showing specific immunolabeling of the ~100k ...
Product cited in: Ip, Fu, Cheng, Tong, Lok, Liang, Ye, Ip: "Anemoside A3 Enhances Cognition through the Regulation of Synaptic Function and Neuroprotection." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 40, Issue 8, pp. 1877-87, 2015

Mao, Xue, Jin, Wang: "Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine." in: Journal of neurochemistry, Vol. 133, Issue 6, pp. 795-805, 2015

Suzuki, Harada, Shiraishi, Kimura: "In vivo pharmacological characterization of TAK-063, a potent and selective phosphodiesterase 10A inhibitor with antipsychotic-like activity in rodents." in: The Journal of pharmacology and experimental therapeutics, Vol. 352, Issue 3, pp. 471-9, 2015

Tassin, Benavides, Plattner, Nishi, Bibb: "Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain." in: The Journal of biological chemistry, Vol. 290, Issue 26, pp. 16319-29, 2015

Mao, Hastings, Fibuch, Wang: "Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice." in: European journal of pharmacology, Vol. 738, pp. 237-44, 2014

Wang, Zhang, Xu, Zhu, Wang, He, Zhang, Guo, Kong, Huang, Li: "Fluoxetine improves behavioral performance by suppressing the production of soluble β-amyloid in APP/PS1 mice." in: Current Alzheimer research, Vol. 11, Issue 7, pp. 672-80, 2014

Bernard, Castano, Bayer, Benke: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." in: Neuropharmacology, Vol. 84, pp. 1-12, 2014

Xue, Edwards, Mao, Guo, Jin, Fibuch, Wang: "Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration." in: Neurochemistry international, Vol. 64, pp. 48-54, 2013

Shen, Fu, Cheng, Fu, Ip: "Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 464-72, 2013

Davis, Olausson, Greengard, Taylor, Nairn: "Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits." in: The European journal of neuroscience, Vol. 35, Issue 2, pp. 300-8, 2012

Ferrario, Loweth, Milovanovic, Wang, Wolf: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, Vol. 490, Issue 3, pp. 180-4, 2011

Kuroiwa, Snyder, Shuto, Fukuda, Yanagawa, Benavides, Nairn, Bibb, Greengard, Nishi: "Phosphodiesterase 4 inhibition enhances the dopamine D1 receptor/PKA/DARPP-32 signaling cascade in frontal cortex." in: Psychopharmacology, 2011

Torres-Altoro, Mathur, Drerup, Thomas, Lovinger, OCallaghan, Bibb: "Organophosphates dysregulate dopamine signaling, glutamatergic neurotransmission, and induce neuronal injury markers in striatum." in: Journal of neurochemistry, Vol. 119, Issue 2, pp. 303-13, 2011

Sears, Liu, Narayanan, Sharf, Yeckel, Laubach, Aghajanian, DiLeone: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, 2010

Santini, Sgambato-Faure, Li, Savasta, Dovero, Fisone, Bezard: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, 2010

Santini, Heiman, Greengard, Valjent, Fisone: "Inhibition of mTOR signaling in Parkinson's disease prevents L-DOPA-induced dyskinesia." in: Science signaling, Vol. 2, Issue 80, pp. ra36, 2009

Rebholz, Nishi, Liebscher, Nairn, Flajolet, Greengard: "CK2 negatively regulates Galphas signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14096-101, 2009

Zheng, Keifer: "PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning." in: Journal of neurophysiology, Vol. 101, Issue 5, pp. 2539-49, 2009

Background publications Sun, Milovanovic, Zhao, Wolf: "Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 16, pp. 4216-30, 2008