NMDA Receptor, NR2B Subunit (0) (pTyr1336) antibody

Antigen: NMDA Receptor, NR2B Subunit (0)

Epitope: pTyr1336

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361484

Additional Information

Alternative name: Phospho-Tyr1336 NMDA Receptor NR2B Subunit

Format: Affinity-purified

Description: The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer?s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003). Anti-Phospho-Tyr1336 NMDA Receptor NR2B Subunit Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1336 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by (-phosphatase treatment.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1336 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Publications

Publications: Jiang, Knox, Pathipati et al.: "Developmental localization of NMDA receptors, Src and MAP kinases in mouse brain." in: Neuroscience letters, Vol. 503, Issue 3, pp. 215-9, 2011 (PubMed).