NMDA NR2B-Subunit (pTyr1472) antibody

Antigen: NMDA NR2B-Subunit

Epitope: pTyr1472

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361485

Additional Information

Alternative name: Phospho-Tyr1472 NMDA NR2B-Subunit

Format: Affinity-purified

Description: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a, Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001, Roche et al., 2001). Anti-Phospho Tyr1472 NMDA NR2B-Subunit Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels prote~65k and ~115k. Immunolabeling is completely blocked by blocked by either (-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Publications

Publications: Castillo, Norcini, Baquero-Buitrago et al.: "The NMDA-evoked cytoplasmic calcium increase in adult rat DRG neuronal somata was potentiated by SP pretreatment in a PKC-dependent manner." in: Neuroscience, 2011 (PubMed).

Venkitaramani, Moura, Picciotto et al.: "Striatal-enriched protein tyrosine phosphatase (STEP) knockout mice have enhanced hippocampal memory." in: The European journal of neuroscience, Vol. 33, Issue 12, pp. 2288-98, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (1:1000).

Carreño, Walch, Dutta et al.: "Brain-Derived Neurotrophic Factor-Tyrosine Kinase B Pathway Mediates NMDA Receptor NR2B Subunit Phosphorylation in the Supraoptic Nuclei Following Progressive Dehydration." in: Journal of neuroendocrinology, Vol. 23, Issue 10, pp. 894-905, 2011 (PubMed).