RAF1 antibody (pSer301)

Catalog No. ABIN361490

The Rabbit Polyclonal anti-RAF1 antibody is suitable to detect RAF1 in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for RAF1 antibody (pSer301) (ABIN361490)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RAF1 Antibody

Binding Specificity: pSer301

Specificity: Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser301. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Human, Rat

Predicted Reactivity: bovine, canine, chicken, mouse, non-human primate, Xenopus

Characteristics: Our Raf-1 (Ser301) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Raf-1 (Ser301) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1

Background:

Synonyms: c Raf antibody, C-raf antibody, C-Raf proto-oncogene serine/threonine kinase antibody, CMD1NN antibody, Craf 1 transforming gene antibody, cRaf antibody, Craf1 transforming gene antibody, EC 2.7.11.1 antibody, kinase Raf1 antibody, Murine sarcoma 3611 oncogene 1 antibody, NS5 antibody, Oncogene MIL antibody, Oncogene RAF1 antibody, OTTHUMP00000160218 antibody, OTTHUMP00000207813 antibody, OTTHUMP00000209389 antibody, Protein kinase raf 1 antibody, Proto-oncogene c-RAF antibody, Raf 1 antibody, Raf 1 proto oncogene serine/threonine kinase antibody, RAF antibody, Raf proto oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, RAF-1 antibody, RAF1 antibody, RAF1_HUMAN antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody, v raf 1 murine leukemia viral oncogene homolog 1 antibody, v-raf murine sarcoma viral oncogene homolog 1 antibody, v-raf-1 murine leukemia viral oncogene-like protein 1 antibody, vraf1 murine leukemia viral oncogene homolog 1 antibody

Description Logic: Our Raf-1 (Ser301) rabbit polyclonal phosphospecific primary antibody is produced . It detects Raf-1 (Ser301) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: RAF1

Molecular Weight: 74 kDa

Gene ID: 24703

UniProt: P11365

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling