Recombinant RAF1 antibody (AA 300-350)

Catalog No. ABIN7795692

The Rabbit Monoclonal anti-RAF1 antibody is suitable to detect RAF1 in samples from Human and Mouse. It has been validated for WB, FACS and IP.

Quick Overview for Recombinant RAF1 antibody (AA 300-350) (ABIN7795692)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: BLR289L

Product Details anti-RAF1 Antibody

Binding Specificity: AA 300-350

Purpose: Rabbit anti-RAF1/c-Raf Recombinant Monoclonal Antibody [BLR289L]

Purification: Purified

Immunogen: Between 300 and 350

Isotype: IgG

Application Details

Application Notes: Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.,IP,6 μL/mg lysate ,WB,11000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1/c-Raf

Background: RAF1 is a mitogen activated kinase kinase kinase (MAPKKK). In response to extracellular signals, RAF1 phosphorylates the MEK1 and MEK2 MAP kinase kinases. MEK1 and MEK2 subsequently phosphorylate and activate the ERK1 and ERK2 MAP kinases to exert pleiotropic effects on cellular functions such as growth, division, differentiation, motility, and apoptosis. RAF1 associates with the Ras family of membrane GTPases and functions downstream of Ras activity.

Gene ID: 5894

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling