Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) antibody

Antigen: Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D)

Synonyms: CD100, SEMAJ, coll-4, C9orf164, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, Semaj, Semcl2, Semacl2

Clonality: Monoclonal (SK-3)

Host: Mouse

Reactivity: Mouse (Murine), Human

Application: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Catalog no.: ABIN361535

Additional Information

Alternative name: Semaphorin 4D

Gene ID: 20354

Immunogen: Recombinant protein of Mouse Semaphorin 4D (extracellular domain)

Format: Affinity-purified

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: SK-3

Description: Semaphorins are a family of phylogeneticallyconserved soluble and transmembrane proteins. Semaphorins were originally identified as axon guidance factors during neuronal development.Semaphorins are now known to be widely expressed mediators that play significantroles in immune responses and organ morphogenesis. Sema4D (also known as CD100) is a 150-kDa transmembrane protein of the class IV semaphorin subfamily. Sema4D is expressed at high levels in lymphoid organs, including the spleen, thymus, and lymph nodes, and in non-lymphoidorgans, such as the brain, heart, andkidney. In lymphoid organs, Sema4D is expressed abundantly by T cells but weakly by B cells. Its expression is significantly enhanced inboth T and B cells, following their respective activation. Sema4D is proteolyticallycleaved from their transmembrane form to generate 120-kDa soluble forms. Two types of receptors, Plexin-B1 and CD72, have been identified for soluble Sema4D.

Application Details

Application Notes: ELISA: 1-10ug/ml. IP: 5ug/ml. FACS: 0.05ug/ml

Concentration: 0.25 mg/ml

Purification: ProteinG Affinity Purified

Buffer: PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]

Storage: Store below -20 degrees Celsius

Research Area: Neurology

Restrictions: For Research Use only

Publications

Publications: Kumanogoh, Watanabe, Lee et al.: "Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling." in: Immunity, Vol. 13, Issue 5, pp. 621-31, 2001 (PubMed).

Kumanogoh, Suzuki, Chng et al.: "Requirement for the lymphocyte semaphorin, CD100, in the induction of antigen-specific T cells and the maturation of dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1175-81, 2002 (PubMed).

Kumanogoh, Shikina, Watanabe et al.: "Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment." in: International immunology, Vol. 17, Issue 10, pp. 1277-82, 2005 (PubMed).