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CAMK2G antibody (Calcium/calmodulin-Dependent Protein Kinase II gamma)

Details for Product anti-CAMK2G Antibody No. ABIN361643, Supplier: Log in to see
Antigen
  • cam2
  • camk2
  • camk2g
  • camkII
  • camkb
  • CaMKII
  • R74975
  • mKIAA0968
  • CAMK
  • CAMK-II
  • CAMKG
  • 5930429P18Rik
  • Camkg
  • calcium/calmodulin-dependent protein kinase II gamma
  • calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2G
  • camk2b
  • Camk2a
  • Camk2g
Alternatives
anti-Human CAMK2G antibody for Western Blotting
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
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80
77
22
4
3
3
3
2
2
1
1
1
Host
Mouse
69
48
Clonality (Clone)
Monoclonal ()
Conjugate
This CAMK2G antibody is un-conjugated
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
112
69
62
53
42
42
19
5
3
2
1
1
1
1
Options
Supplier
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Immunogen Partially purified rat CaMKII
Clone 6G9
Isotype IgG1
Specificity Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Sensitivity 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Purification Protein G Purified
Alternative Name CaMKII (CAMK2G Antibody Abstract)
Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Gene ID 12322
NCBI Accession NP_033922
UniProt P11798
Research Area Cancer, Hypertrophy, Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction
Application Notes
  • WB (1:10000)
  • IHC (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-CAMK2G antibody (Calcium/calmodulin-Dependent Protein Kinase II gamma) (ABIN361643) CamKII (6G9) in cultured cortical neurons.
Western Blotting (WB) image for anti-CAMK2G antibody (Calcium/calmodulin-Dependent Protein Kinase II gamma) (ABIN361643) western rat membrane 1 in 1000 CaMKII.
Product cited in: Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010 (PubMed).

Background publications Hughes, Edin, Antonsson, Grundström: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001 (PubMed).

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000 (PubMed).

Leonard, Lim, Hemsworth, Horne, Hell: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999 (PubMed).

Makhinson, Chotiner, Watson, ODell: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999 (PubMed).

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999 (PubMed).

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998 (PubMed).

Barria, Muller, Derkach, Griffith, Soderling: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997 (PubMed).

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997 (PubMed).

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995 (PubMed).

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994 (PubMed).

Nghiem, Saati, Martens, Gardner, Schulman: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993 (PubMed).

Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987 (PubMed).