Cu/Zn Superoxide Dismutase (SOD) antibody

Antigen: Cu/Zn Superoxide Dismutase (SOD)

Synonyms: LOC692639, ALS, SOD, ALS1, IPOA, homodimer

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Monkey, Coral, Dog (Canine), Hamster, Pig (Porcine), Rabbit, Sheep (Ovine), Xenopus laevis

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361645

Additional Information

Alternative name: SOD1

Immunogen: Human Cu/Zn SOD

Format: Affinity-purified

Description: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2

Specificity: Detects 23kDa (human) and 19kDa (other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots. Species cross-reactivity: Human, Rat, Mouse, Bovine, Monkey, Coral, Dog, Hamster, Pig, Rabbit, Sheep, Xenopus.

Application Details

Application Notes: 0.2ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of HeLa cell lysate

Concentration: 1.0mg/ml

Buffer: Affinity (antigen) purified. PBS (pH7.0), 0.1% NaN3, 50% glycerol.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Restrictions: For Research Use only

Images

CuZn SOD (human), human placenta. Courtesy of Joan Telfer, University of Glasgow

Publications

Publications: Adachi, Ohta, Yamada et al.: "Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 212, Issue 3, pp. 89-102, 1993 (PubMed).

Wispue, Clark, Burhans et al.: "Synthesis and processing of the precursor for human mangano-superoxide dismutase." in: Biochimica et biophysica acta, Vol. 994, Issue 1, pp. 30-6, 1989 (PubMed).

Hassan: "Biosynthesis and regulation of superoxide dismutases." in: Free radical biology & medicine, Vol. 5, Issue 5-6, pp. 377-85, 1989 (PubMed).

Oikawa, Ito, Ashino et al.: "Radicicol, a microbial cell differentiation modulator, inhibits in vivo angiogenesis." in: European journal of pharmacology, Vol. 241, Issue 2-3, pp. 221-7, 1994 (PubMed).

Shinder, Lacourse, Minotti et al.: "Mutant Cu/Zn-superoxide dismutase proteins have altered solubility and interact with heat shock/stress proteins in models of amyotrophic lateral sclerosis." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12791-6, 2001 (PubMed).

Gao, Flores, Leff et al.: "Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 284, Issue 6, pp. L917-25, 2003 (PubMed).