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HSP90AA2 antibody (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 2) (AA 604-731)

Details for Product anti-HSP90AA2 Antibody No. ABIN361714, Supplier: Log in to see
Antigen
  • fb17b01
  • hsp90
  • hsp90a
  • hsp90a.1
  • hsp90alpha
  • wu:fb17b01
  • zgc:86652
  • HSP90ALPHA
  • HSPCA
  • HSPCAL3
Epitope
AA 604-731
17
13
13
13
6
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
86
82
81
6
6
6
6
4
4
3
2
2
2
2
2
2
2
Host
Mouse
46
45
2
Clonality (Clone)
Monoclonal ()
Conjugate
This HSP90AA2 antibody is un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
68
47
44
24
22
20
19
8
2
1
Supplier
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Immunogen Recombinant human HSP90alpha, Specificity mapped to amino acids 604-731
Clone Hyb-K41009
Specificity Detects 90 kDa. This is an alpha-specific product.
Sensitivity 1 µg/mL of SMC-108 was sufficient for detection of Hsp90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Alternative Name HSP90 alpha (HSP90AA2 Antibody Abstract)
Background HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer.(2) From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, HSP90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9).
Cellular Localization: Cytoplasm | Melanosome
Gene ID 3320
NCBI Accession NP_001017963
UniProt P07900
Research Area Cancer, Heat Shock Proteins
Application Notes Recommended Dilution: WB (1:1000), IHC (1:5000)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-HSP90AA2 antibody (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 2) (AA 604-731) (ABIN361714) Hsp90alpha (K41009) Mouse Colon Cancer 1 IN 5,000, Amplifier.
Western Blotting (WB) image for anti-HSP90AA2 antibody (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 2) (AA 604-731) (ABIN361714) Total Rat Tissue Hsp90alpha WB 1 in 1000.
Product cited in: Ishikawa, Sakurai: "Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells." in: The FEBS journal, Vol. 282, Issue 2, pp. 332-40, 2015 (PubMed).

Xi, Hu, Buckhaults et al.: "Heat shock factor Hsf1 cooperates with ErbB2 (Her2/Neu) protein to promote mammary tumorigenesis and metastasis." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35646-57, 2012 (PubMed).

Quanz, Herbette, Sayarath et al.: "Heat shock protein 90α (Hsp90α) is phosphorylated in response to DNA damage and accumulates in repair foci." in: The Journal of biological chemistry, Vol. 287, Issue 12, pp. 8803-15, 2012 (PubMed).

Shipp, Watson, Jones: "Associations of HSP90 client proteins in human breast cancer." in: Anticancer research, Vol. 31, Issue 6, pp. 2095-101, 2011 (PubMed).

Background publications Arlander, Eapen, Vroman et al.: "Hsp90 inhibition depletes Chk1 and sensitizes tumor cells to replication stress." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52572-7, 2003 (PubMed).

Pratt, Toft: "Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery." in: Experimental biology and medicine (Maywood, N.J.), Vol. 228, Issue 2, pp. 111-33, 2003 (PubMed).

Neckers: "Hsp90 inhibitors as novel cancer chemotherapeutic agents." in: Trends in molecular medicine, Vol. 8, Issue 4 Suppl, pp. S55-61, 2002 (PubMed).

Pearl, Prodromou: "Structure, function, and mechanism of the Hsp90 molecular chaperone." in: Advances in protein chemistry, Vol. 59, pp. 157-86, 2002 (PubMed).

Pratt: "The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors." in: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), Vol. 217, Issue 4, pp. 420-34, 1998 (PubMed).

Nemoto, Sato, Iwanari et al.: "Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis." in: The Journal of biological chemistry, Vol. 272, Issue 42, pp. 26179-87, 1997 (PubMed).

Nemoto, Roi, Matsusaka et al.: "Isoform-specific monoclonal antibodies against HSP90." in: Biochemistry and molecular biology international, Vol. 42, Issue 5, pp. 881-9, 1998 (PubMed).

Pratt, Toft: "Steroid receptor interactions with heat shock protein and immunophilin chaperones." in: Endocrine reviews, Vol. 18, Issue 3, pp. 306-60, 1997 (PubMed).

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997 (PubMed).

Whitesell, Mimnaugh, De Costa et al.: "Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for stress proteins in oncogenic transformation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 18, pp. 8324-8, 1994 (PubMed).

Minami, Kawasaki, Miyata et al.: "Analysis of native forms and isoform compositions of the mouse 90-kDa heat shock protein, HSP90." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10099-103, 1991 (PubMed).

Garg, Hassid: "Nitric oxide decreases cytosolic free calcium in Balb/c 3T3 fibroblasts by a cyclic GMP-independent mechanism." in: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 9-12, 1991 (PubMed).