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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibody

Antigen

DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)

Synonyms
AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHY ... show more
Clonality Monoclonal (60B1220-1)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human, Mouse (Murine), Zebrafish

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChiP)
6 references available
Catalog no. ABIN361726
Quantity 100ug  (0.5mg/mL)
Price 357.50 $   Plus shipping costs $45.00
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Additional Information

Alternative name DNMT1
Gene ID 1786
UniProt P26358
Immunogen Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1 (Gene accession number NP_001370)
Isotype IgG1 kappa
Clone 60B1220-1
Description Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Synonyms: DNA Mtase, DNMT, MCMT, DNA methyltransferase 1
Characteristics Accession Number: NP_001370
Specificity It will cross-react with mouse DNMT1.
Synonyms AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Application Details

Application Notes 2-4ug/mL (WB), 1-2ug/mL (IHC)
Concentration 0.5mg/mL
Purification Protein G Purified
Buffer PBS
Preservative 0.05% BSA
Storage Store at -20° C. Shipping Conditions: Blue Ice or 4° C
Research Area Cell Signaling, Organelles, DNA/RNA
Restrictions For Research Use only

Publications

Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Bestor, Laudano, Mattaliano et al.: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

Alternatives

Alternatives for antigen "DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)", type "Antibodies"
Hosts Rabbit (81), Mouse (8), Goat (7), Chicken (5), Rat (1)
Reactivities Human (94), Dog (Canine) (39), Mouse (Murine) (35), Pig (Porcine) (34), Cow (Bovine) (32), Sheep (Ovine) (28), Rat (Rattus) (26), Chicken (23), Horse (Equine) (23), Zebrafish (2)
Applications Western Blotting (WB) (47), Immunofluorescence (IF) (40), ELISA (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (16), Dot Blot (Dot) (6), Immunohistochemistry (IHC) (5), Chromatin Immunoprecipitation (ChiP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr399 (8), AA 637-650 (5), pSer153 (5), pSer730 (5), pSer714 (4), pTyr985 (4), AA 177-550 (3), N-Term (3), pSer154 (3), pTyr405 (3), pTyr969 (3), C-Term (2), Ser1105 (2), AA 1603-1616 (1), AA 82-148 (1), C-Term,Ser1602 (1), Internal Region (1), pSer509 (1), pSer671 (1)