DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibody

Antigen: DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Clonality: Monoclonal (60B1220-1)

Host: Mouse

Reactivity: Human, Mouse (Murine), Zebrafish (Danio rerio)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChiP)

Catalog no.: ABIN361726

Additional Information

Alternative name: DNMT1

Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1 (Gene accession number NP_001370)

Format: Protein G purified

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 60B1220-1

Description: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Specificity: It will cross react with mouse DNMT1. Species Cross-reactivity Human, Mouse, Zebrafish

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Application Details

Application Notes: 2-4ug/mL for Western blot, 1-2ug/mL for IHC

Concentration: 0.5mg/mL

Buffer: Protein G purified, formatted in PBS containing 0.05% BSA and 0.05% sodium azide

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: DNA/RNA

Restrictions: For Research Use only

Images

DNMT1 (60B1220), human medullar kidney tissue

DNMT1 (60B1220.1), recombinant

DNMT1 (60B1220.1), Mouse brain tissue 1 in 10000, Amplifier

Publications

Publications: Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Bestor, Laudano, Mattaliano et al.: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).