HSP70 antibody (Heat Shock Protein 70)

Details for Product anti-HSP70 Antibody No. ABIN361735, Supplier: Log in to see
Antigen
  • HSP70
  • HEAT SHOCK PROTEIN 70
  • HEAT SHOCK PROTEIN 70-7
  • HSC70-7
  • K9P8.5
  • K9P8_5
  • chloroplast heat shock protein 70-2
  • cpHsc70-2
  • F19K16.12
  • F19K16_12
  • LOC100305036
  • hsc70
  • CG31354
  • Hsp70Bb
  • hsp70B
  • hsp70Bb-prime
  • DmelCG5834
  • CG5834
  • APG-2
  • HS24/P52
  • HSPH2
  • RY
  • hsp70
  • hsp70RY
  • hsc71
  • Hsp70
  • Hsp70-1
  • Hsp70.1
  • hsp68
  • Hsp110
  • irp94
  • HSPA1
  • HSP70B'
  • HSPA6
  • ARABIDOPSIS HEAT SHOCK PROTEIN 70
  • ATHSP70
  • heat shock protein 70
  • hsp70-5
  • heat shock 70 kD protein cognate
  • heat shock protein 70-2
  • Heat shock protein 70
  • heat shock protein 70
  • CG5834 gene product from transcript CG5834-RA
  • heat shock 70kDa protein 1A
  • heat shock 70kDa protein 2
  • heat shock 70kDa protein 4
  • heat shock cognate protein 70a
  • heat shock protein 1B
  • heat shock protein 4
  • heat shock protein 70-4
  • heat shock protein1
  • Protein HSP-70
  • LOC692373
  • CPHSC70-2EAT SHOCK PROTEIN 70-2
  • AT1G79920
  • HSP70
  • PCC7424_2419
  • Isop_1041
  • CGB_C3390W
  • Bacsa_1698
  • dnaK-B
  • LOC100305036
  • Hsp70Bbb
  • HSPA1A
  • HSPA2
  • HSPA4
  • hsc70a
  • Hspa1b
  • Hspa4
  • hsp1
  • hsp-70
Reactivity
Amphibian, Chicken, Fish, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Yeast
624
424
423
197
184
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149
114
110
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96
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75
58
54
50
39
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8
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2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
380
312
40
18
Clonality (Clone)
Monoclonal ()
Conjugate
This HSP70 antibody is un-conjugated
35
35
25
24
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11
9
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2
Application
Antibody Array (AA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
662
291
283
275
255
235
148
109
72
62
50
26
18
11
9
4
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Human recombinant HSP70 overexpressed in E.coli
Clone 5A5
Isotype IgG1
Specificity Detects ~70 kDa. May detect HSP70, HSC70, Grp78 and HSP72.
Purification Protein G Purified
Alternative Name HSP70 (HSP70 Antibody Abstract)
Background HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
Gene ID 3303
NCBI Accession NP_005336
UniProt P08107
Research Area Heat Shock Proteins
Application Notes
  • WB (1:1000)
  • ICC/IF (1:500)
  • IP (1 μg)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-162 was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Heat Shock Protein 70 (HSP70) antibody (ABIN361735) Hsp70 (5A5), rat lysate
Background publications Balashova, Chang, Lamothe, Sun, Beuve: "Characterization of a novel type of endogenous activator of soluble guanylyl cyclase." in: The Journal of biological chemistry, Vol. 280, Issue 3, pp. 2186-96, 2005 (PubMed).

Fink: "Chaperone-mediated protein folding." in: Physiological reviews, Vol. 79, Issue 2, pp. 425-49, 1999 (PubMed).

Boorstein, Ziegelhoffer, Craig: "Molecular evolution of the HSP70 multigene family." in: Journal of molecular evolution, Vol. 38, Issue 1, pp. 1-17, 1994 (PubMed).

Bork, Sander, Valencia: "An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7290-4, 1992 (PubMed).

Rothman: "Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells." in: Cell, Vol. 59, Issue 4, pp. 591-601, 1990 (PubMed).

DeLuca-Flaherty, McKay, Parham, Hill: "Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis." in: Cell, Vol. 62, Issue 5, pp. 875-87, 1990 (PubMed).

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