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CD74 antibody (HLA-DR-gamma)

Details for Product anti-CD74 Antibody No. ABIN361789, Supplier: Log in to see
Antigen
  • CLIP
  • DHLAG
  • HLADG
  • Ia-GAMMA
  • Ii
  • II
  • INVG34
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • Cd74 molecule, major histocompatibility complex, class II invariant chain
  • Cd74
  • CD74
Alternatives
anti-Human CD74 antibody for Flow Cytometry
Reactivity
Human, Mouse (Murine)
405
106
24
22
1
1
1
Host
Mouse
366
41
19
6
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is un-conjugated
24
23
17
15
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13
13
12
12
10
9
8
8
8
8
8
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7
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
297
231
163
133
128
113
94
74
64
13
11
4
3
3
2
1
1
1
1
1
1
Options
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Immunogen Human CD74 invariant chain synthetic peptide
Clone PIN-1
Isotype IgG
Specificity Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Sensitivity 1 µg/mL was sufficient for detection of SMC-116 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG:AP as the secondary antibody.
Purification Protein G Purified
Alternative Name CD74 (CD74 Antibody Abstract)
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Gene ID 972
NCBI Accession NP_001020329
UniProt P04233
Research Area Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-116 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-CD74 antibody (HLA-DR-gamma) (ABIN361789) CD 74 (PIN 1 1) N87 lysates mixed with Macrophage inhibitory factor.
Product cited in: Schneppenheim, Hüttl, Kruchen, Fluhrer, Müller, Saftig, Schneppenheim, Martin, Schröder: "Signal-peptide-peptidase-like 2a is required for CD74 intramembrane proteolysis in human B cells." in: Biochemical and biophysical research communications, Vol. 451, Issue 1, pp. 48-53, 2014 (PubMed).

Katsel, Tan, Haroutunian: "Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals." in: PLoS ONE, Vol. 4, Issue 10, pp. e7642, 2009 (PubMed).

Background publications Starlets, Gore, Binsky, Haran, Harpaz, Shvidel, Becker-Herman, Berrebi, Shachar: "Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival." in: Blood, Vol. 107, Issue 12, pp. 4807-16, 2006 (PubMed).

Barrera, Beswick, Sierra, Bland, Espejo, Mifflin, Adegboyega, Crowe, Ernst, Reyes: "Polarized expression of CD74 by gastric epithelial cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 53, Issue 12, pp. 1481-9, 2005 (PubMed).

Becker-Herman, Arie, Medvedovsky, Kerem, Shachar: "CD74 is a member of the regulated intramembrane proteolysis-processed protein family." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5061-9, 2005 (PubMed).

Burton, Ely, Reddy, Stein, Gold, Cardillo, Goldenberg: "CD74 is expressed by multiple myeloma and is a promising target for therapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6606-11, 2004 (PubMed).

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, 1997 (PubMed).

Denzin, Robbins, Carboy-Newcomb, Cresswell: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995 (PubMed).