Lysine (lys) (acetylated) antibody
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- Target
- Lysine (lys)
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Binding Specificity
- acetylated
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)
- Specificity
- Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
- Purification
- Protein G Purified
- Immunogen
- Acetylated KLH
- Clone
- 7F8
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg of ABIN361802 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 μg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- Lysine (lys)
- Alternative Name
- Lysine
- Synonyms
- lysine antibody, lys antibody
- Target Type
- Amino Acid
- Background
- Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
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