Acetylated Lysine antibody

Details for Product No. ABIN361802
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Antigen
Reactivity
Chemical
(3), (2), (2), (2), (1), (1), (1)
Host
Mouse
(4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(7), (6), (4), (4), (3), (3)
Pubmed 5 references available
Quantity 25 μg
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Immunogen Acetylated KLH
Clone 7F8
Specificity Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
Sensitivity 1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
Cellular Localization: Nucleus
Research Area Cell Signaling
Application Notes Recommended Dilution: WB (1:1000)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Acetylated Lysine antibody Acetylated Lysine (7F8), mouse backskin
Background publications Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).

Yang: "Lysine acetylation and the bromodomain: a new partnership for signaling." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 10, pp. 1076-87, 2004 (PubMed).

Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Hassig, Schreiber: "Nuclear histone acetylases and deacetylases and transcriptional regulation: HATs off to HDACs." in: Current opinion in chemical biology, Vol. 1, Issue 3, pp. 300-8, 1998 (PubMed).

General Hughes: "Polyglutamine disease: acetyltransferases awry." in: Current biology : CB, Vol. 12, Issue 4, pp. R141-3, 2002 (PubMed).

Validation Images
Catalog No. ABIN361802
217.80 $
Plus shipping costs $45.00
Quantity
Price
25 μg
217.80 $

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