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MEK2 antibody (pThr394)

MAP2K2 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361887
  • Target See all MEK2 (MAP2K2) Antibodies
    MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
    Binding Specificity
    • 19
    • 16
    • 13
    • 11
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr394
    Reactivity
    • 135
    • 81
    • 46
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 110
    • 24
    • 1
    • 1
    Rabbit
    Clonality
    • 110
    • 26
    Polyclonal
    Conjugate
    • 72
    • 11
    • 11
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This MEK2 antibody is un-conjugated
    Application
    • 110
    • 61
    • 36
    • 22
    • 21
    • 20
    • 19
    • 15
    • 14
    • 10
    • 7
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Specificity
    The antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pThr394 (P-G-T (p) -P-T) derived from Human MEK-2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
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  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target
    MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
    Alternative Name
    MEK-2 (MAP2K2 Products)
    Synonyms
    CFC4 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, AA589381 antibody, MK2 antibody, Prkmk2 antibody, ATMKK2 antibody, F27B13.50 antibody, F27B13_50 antibody, MAP KINASE KINASE 1 antibody, MAP kinase kinase 2 antibody, MK1 antibody, map2k2 antibody, wu:fa56c06 antibody, zgc:123171 antibody, zgc:172250 antibody, mitogen-activated protein kinase kinase 2 antibody, mitogen activated protein kinase kinase 2 antibody, MAP kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2a antibody, mitogen-activated protein kinase kinase 2b antibody, MAP2K2 antibody, Map2k2 antibody, MKK2 antibody, map2k2a antibody, map2k2b antibody
    Background
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
    Molecular Weight
    44 kDa
    NCBI Accession
    NP_109587
    UniProt
    P36507
    Pathways
    MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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