ATF2 antibody (pThr51, pThr69)

Catalog No. ABIN361931

The Rabbit Polyclonal anti-ATF2 antibody is suitable to detect ATF2 in samples from Human, Mouse and Rat. It has been validated for IHC and IF.

Quick Overview for ATF2 antibody (pThr51, pThr69) (ABIN361931)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ATF2 Antibody

Binding Specificity: pThr51, pThr69

Specificity: The antibody detects endogenous level of ATF-2 only when phosphorylated at threonine 69 or 51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr69 or 51 (D-Q-T (p) -P-T) derived from Human ATF2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF2

Background: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Molecular Weight: 65-75 kDa

NCBI Accession: NP_001871

UniProt: P15336

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades