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Tumor Protein P53 (TP53) (pSer46) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Epitope
Alternatives

pSer46
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), Immunofluorescence (IF)
Catalog no. ABIN362055
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name p53 (Phospho-Ser46)
Swiss-Prot P04637
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanp53 around the phosphorylation site of serine 46 (M-L-S P -P-D).
Isotype IgG  (Matching secondary antibodies)
Specificity P53 (Phospho-Ser46) (Human) antibody detects endogenous levels of p53 only whenphosphorylated at serine 46.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes WB: 1:500~1:1000 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (302), Mouse (102), Sheep (3)
Reactivities Human (390), Mouse (Murine) (68), Rat (Rattus) (62), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Dog (Canine) (3), Hamster (3), Horse (Equine) (3), Primate (2), Primates (2), Sheep (Ovine) (2), Bird (Avian) (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (337), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (112), Immunohistochemistry (IHC) (81), Immunofluorescence (IF) (75), ELISA (68), Enzyme Immunoassay (EIA) (68), Immunoprecipitation (IP) (53), Flow Cytometry (FACS) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (10), ELISA (Detection) (5), Transfected cell culture (TCC) (3), Chromatin Immunoprecipitation (ChiP) (2), Radioimmunoassay (RIA) (2)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer315 (12), pSer37 (12), pSer33 (10), pSer46 (10), pThr18 (10), pSer6 (9), pSer392 (8), pSer15 (7), pSer20 (7), AA 371-380 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), pSer9 (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer376 (2), pSer378 (2), pThr55 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), acLys120 (1), acLys382 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), phospho (1)