ATM antibody (pSer1981)

Catalog No. ABIN362099

There are 4+ publications for this product available. The Rabbit Polyclonal anti-ATM antibody is suitable to detect ATM in samples from Human and Mouse. It has been validated for IHC.

Quick Overview for ATM antibody (pSer1981) (ABIN362099)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ATM Antibody

Binding Specificity: pSer1981

Specificity: The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer1981 (E-G-S (p) -Q-S) derived from Human ATM. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100  

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-ATM antibody (ABIN362099)

Inaba, Kuboniwa, Sugita, Lamont, Amano: "Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts." in: Infection and immunity, Vol. 80, Issue 8, pp. 2847-57, (2012) (PubMed).

Mahalingam, Tay, Tan, Chai, Wang: "Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts." in: The FEBS journal, Vol. 278, Issue 19, pp. 3724-38, (2011) (PubMed).

Leemput, Masson, Bigot, Errachid, Dansault, Provost, Gadin, Aoufouchi, Menasche, Abitbol: "ATM localization and gene expression in the adult mouse eye." in: Molecular vision, Vol. 15, pp. 393-416, (2009) (PubMed).

Kang, Guo, Tan, Zhao, Tang, Lu: "Expression status of ataxia-telangiectasia-mutated gene correlated with prognosis in advanced gastric cancer." in: Mutation research, Vol. 638, Issue 1-2, pp. 17-25, (2008) (PubMed).

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 350 kDa

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus