ATM antibody (pSer1981)

Catalog No. ABIN6656104

Product Details anti-ATM Antibody

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Binding Specificity: AA 1974-1988, pSer1981

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.   By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000, whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145.  Reactivity is observed against human ATM.  Cross reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM.

Immunogen:

Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Immunogen Type: Peptide

Clone: 10H11-E12

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: Not Recommended

Application Note: Protein A Purified Mab anti-ATM has been tested by ELISA, Flow Cytometry, IF, and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells.  By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts.  This antibody is not recommended for immunohistochemistry.  Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).

ELISA Dilution: 1:20,000 - 1:100,000

Flow Cytometry Dilution: 5 μg/mL

Western Blot Dilution: 1:200 - 1:2,000

IF Microscopy Dilution: 1:100 - 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store Anti-ATM phospho S1981 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-ATM antibody (ABIN6656104)

Chen, Chen, Huang, Gu, Qiu, Qian, Shao, Zhang, Hu, Li, He, Zhou, Abdel-Wahab, Zhang, Fu: "The Augmented R-Loop Is a Unifying Mechanism for Myelodysplastic Syndromes Induced by High-Risk Splicing Factor Mutations." in: Molecular cell, Vol. 69, Issue 3, pp. 412-425.e6, (2019) (PubMed).

Li, Mahon, Sweeney, Verschueren, Kantamani, Li, Hennigs, Marciano, Diebold, Abu-Halawa, Elliott, Sa, Guo, Wang, Cao, Guignabert, Sollier, Nickel, Kaschwich, Cimprich, Rabinovitch: "PPARγ Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis." in: Cell reports, Vol. 26, Issue 5, pp. 1333-1343.e7, (2019) (PubMed).

Parisotto, Grelet, El Bizri, Dai, Terzic, Eckert, Gargowitsch, Bornert, Metzger: "PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo." in: The Journal of experimental medicine, Vol. 215, Issue 6, pp. 1749-1763, (2019) (PubMed).

Ashraf, Hamidullah, Hasanain, Pandey, Maheshwari, Singh, Siddiqui, Konwar, Sashidhara, Sarkar: "Coumarin-chalcone hybrid instigates DNA damage by minor groove binding and stabilizes p53 through post translational modifications." in: Scientific reports, Vol. 7, pp. 45287, (2018) (PubMed).

Gursoy-Yuzugullu, Carman, Serafim, Myronakis, Valente, Price: "Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity." in: Oncotarget, Vol. 8, Issue 15, pp. 24518-24532, (2018) (PubMed).

Vadnais, Chen, Fraszczak, Yu, Boulais, Pinder, Frank, Khandanpour, Hébert, Dellaire, Côté, Richard, Orthwein, Drobetsky, Möröy: "GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1." in: Nature communications, Vol. 9, Issue 1, pp. 1418, (2018) (PubMed).

Valdez, Li, Murray, Liu, Nieto, Champlin, Andersson: "The PARP inhibitor olaparib enhances the cytotoxicity of combined gemcitabine, busulfan and melphalan in lymphoma cells." in: Leukemia & lymphoma, Vol. 58, Issue 11, pp. 2705-2716, (2018) (PubMed).

Chen, Cao, Zhang, Gu, Zhou, Li, Li, Tan, Zeng: "LRRK2 interacts with ATM and regulates Mdm2-p53 cell proliferation axis in response to genotoxic stress." in: Human molecular genetics, Vol. 26, Issue 22, pp. 4494-4505, (2018) (PubMed).

Göder, Emmerich, Nikolova, Kiweler, Schreiber, Kühl, Imhof, Christmann, Heinzel, Schneider, Krämer: "HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130." in: Nature communications, Vol. 9, Issue 1, pp. 764, (2018) (PubMed).

Higo, Naito, Sumida, Shibamoto, Okada, Nomura, Nakagawa, Yamaguchi, Sakai, Hashimoto, Kuramoto, Ito, Hikoso, Akazawa, Lee, Shiojima, McKinnon, Sakata, Komuro: "DNA single-strand break-induced DNA damage response causes heart failure." in: Nature communications, Vol. 8, pp. 15104, (2018) (PubMed).

Fujiwara, Muramatsu, Nishii, Tokunaka, Tahara, Ueno, Yamori, Sugimoto, Seimiya: "Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells." in: Scientific reports, Vol. 8, Issue 1, pp. 14827, (2018) (PubMed).

Zhai, Steinø, Bacha, Brown, Daugaard: "Dianhydrogalactitol induces replication-dependent DNA damage in tumor cells preferentially resolved by homologous recombination." in: Cell death & disease, Vol. 9, Issue 10, pp. 1016, (2018) (PubMed).

Inoue, Li, Tseng, Beerman, Elia, Bendall, Lemonnier, Kron, Cescon, Hao, Lind, Takayama, Planello, Shen, Shih, Larsen, Li, Snow, Wakeham, Haight, Gorrini, Bassi, Thu, Murakami, Elford, Ueda, Straley et al.: "Mutant IDH1 Downregulates ATM and Alters DNA Repair and Sensitivity to DNA Damage Independent of TET2. ..." in: Cancer cell, Vol. 30, Issue 2, pp. 337-348, (2017) (PubMed).

Zheng, Zhu, Zhao, Yao, Wu, Sun: "Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation." in: Molecular medicine reports, Vol. 15, Issue 1, pp. 375-379, (2017) (PubMed).

Feng, Jasin: "BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination." in: Nature communications, Vol. 8, Issue 1, pp. 525, (2017) (PubMed).

Michelini, Pitchiaya, Vitelli, Sharma, Gioia, Pessina, Cabrini, Wang, Capozzo, Iannelli, Matti, Francia, Shivashankar, Walter, dAdda di Fagagna: "Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks." in: Nature cell biology, Vol. 19, Issue 12, pp. 1400-1411, (2017) (PubMed).

Wangondu, Teal, Park, Heston, Delecluse, Miller: "DNA Damage Signaling Is Induced in the Absence of Epstein-Barr Virus (EBV) Lytic DNA Replication and in Response to Expression of ZEBRA." in: PLoS ONE, Vol. 10, Issue 5, pp. e0126088, (2016) (PubMed).

Modzelewski, Hilz, Crate, Schweidenback, Fogarty, Grenier, Freire, Cohen, Grimson: "Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males." in: Journal of cell science, Vol. 128, Issue 12, pp. 2314-27, (2016) (PubMed).

Torres, Pandita, Kulak, Kumar, Formstecher, Horikoshi, Mujoo, Hunt, Zhao, Lum, Zaman, Yeaman, White, Pandita: "Role of the Exocyst Complex Component Sec6/8 in Genomic Stability." in: Molecular and cellular biology, Vol. 35, Issue 21, pp. 3633-45, (2016) (PubMed).

Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, (2016) (PubMed).

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM (ATM Products)

Synonyms: ATM antibody, Atm antibody, CG6535 antibody, Dmel\\CG6535 antibody, Tefu antibody, atm antibody, atm/tefu antibody, dATM antibody, tef antibody, Xatm antibody, at1 antibody, atdc antibody, tel1 antibody, telo1 antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, TEL1 antibody, TELO1 antibody, AI256621 antibody, C030026E19Rik antibody, telomere fusion antibody, ATM serine/threonine kinase L homeolog antibody, ATM serine/threonine kinase antibody, ataxia telangiectasia mutated antibody, ataxia telangiectasia mutated (atm) antibody, serine/threonine-protein kinase ATM antibody, tefu antibody, atm.L antibody, atm antibody, ATM antibody, EDI_100660 antibody, CpipJ_CPIJ001772 antibody, BDBG_08252 antibody, PAAG_02532 antibody, MCYG_05088 antibody, VDBG_06833 antibody, ACLA_015700 antibody, LOC5565620 antibody, MGYG_07634 antibody, PGTG_14279 antibody, Atm antibody

Background:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Gene ID: 472

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus