LIM Domain Kinase 1 antibody (pThr508)
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- Target See all LIM Domain Kinase 1 (LIMK1) Antibodies
- LIM Domain Kinase 1 (LIMK1)
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Binding Specificity
- pThr508
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LIM Domain Kinase 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pThr508 (R-Y-T (p) -V-V) derived from Human LIMK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
- Top Product
- Discover our top product LIMK1 Primary Antibody
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- Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, (2009) (PubMed).
: "
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DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, (2009) (PubMed).
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- Target
- LIM Domain Kinase 1 (LIMK1)
- Alternative Name
- LIMK1 (LIMK1 Products)
- Synonyms
- limk1 antibody, xlimk1 antibody, GB16654 antibody, LIMK1 antibody, LIMK antibody, LIMK-1 antibody, LIM domain kinase 1 antibody, LIM domain kinase 1a antibody, LIM domain kinase 1 L homeolog antibody, LIM-domain containing, protein kinase antibody, LIMK1 antibody, limk1a antibody, limk1 antibody, CpipJ_CPIJ000717 antibody, LOC413152 antibody, Limk1 antibody, limk1.L antibody
- Background
- Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
- Molecular Weight
- 72 kDa
- NCBI Accession
- NP_002305
- UniProt
- P53667
- Pathways
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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