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Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody

Antigen

Insulin Receptor Substrate 1 (IRS1)
Synonyms HIRS-1, G972R, IRS-1, IRS1IRM, irs1, MGC75645, IRS1
Epitope
Alternatives

pSer312
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN362133
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name IRS-1 (Phospho-Ser312)
Swiss-Prot P35568
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-S P -P-A).
Isotype IgG  (Matching secondary antibodies)
Specificity IRS-1 (Phospho-Ser312) (Human) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes WB: 1:500~1:1000
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Insulin Receptor Substrate 1 (IRS1)", type "Antibodies"
Hosts Rabbit (165), Mouse (4)
Reactivities Human (150), Mouse (Murine) (107), Rat (Rattus) (95), Cow (Bovine) (5), Chicken (3), Dog (Canine) (3), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (100), Immunofluorescence (IF) (65), Immunohistochemistry (IHC) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Flow Cytometry (FACS) (28), ELISA (25), Enzyme Immunoassay (EIA) (14), Immunoprecipitation (IP) (12), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer307 (19), pSer312 (15), pSer639 (14), pSer636 (11), pTyr896 (4), pSer612 (3), pSer616 (2), pTyr1179 (2), pTyr612 (2), pS307 (1), pTyr1225 (1), pTyr941 (1), pY1179 (1), pY896 (1)